Protein expression and purification

Heterologous expression in Escherichia coli of Neurospora crassa neutral trehalase as an active enzyme

Gene expression / Multidisciplinary / Neurospora crassa / Biological Sciences / Vancomycin / Aquaporins (AQP) / Mutagenesis / Copper / Escherichia coli / Animals / Polymerase Chain Reaction / Temperature / Phosphorylation / Biochemical / Affinity chromatography / Enzyme / CHEMICAL SCIENCES / Cattle / Molecular cloning / Protein expression and purification / Fluorescent Antibody Technique / Anti-Bacterial Agents / Active site / Rats / Specific Activity / Time Factors / Filamentous fungi / Protein Expression / Metal ion / Amino Acid Sequence / Base Sequence / Recombinant Protein / Heterologous Expression / Hydrogen-Ion Concentration / Site-directed Mutagenesis / Glycosyl Hydrolases / Biochemistry and cell biology / Molecular Sequence Data / Aquaporins (AQP) / Mutagenesis / Copper / Escherichia coli / Animals / Polymerase Chain Reaction / Temperature / Phosphorylation / Biochemical / Affinity chromatography / Enzyme / CHEMICAL SCIENCES / Cattle / Molecular cloning / Protein expression and purification / Fluorescent Antibody Technique / Anti-Bacterial Agents / Active site / Rats / Specific Activity / Time Factors / Filamentous fungi / Protein Expression / Metal ion / Amino Acid Sequence / Base Sequence / Recombinant Protein / Heterologous Expression / Hydrogen-Ion Concentration / Site-directed Mutagenesis / Glycosyl Hydrolases / Biochemistry and cell biology / Molecular Sequence Data

Cloning, expression, purification, and characterization of Vibrio cholerae transcriptional activator, HlyU

Technology / High Pressure / Biotechnology / Protein / Glycobiology / Cytotoxicity / Biological Sciences / Escherichia coli / Bacteria / Physical sciences / Molecular Biotechnology / Expression / Affinity chromatography / Ion Exchange Chromatography / Enzyme / Protein expression and purification / Enzyme activity / Amino Acid Profile / Molecular Mass / Molecular weight / Protein Kinase / Amino Acid Sequence / Open Reading Frame / Reaction Mechanism / Recombinant Protein / Polyacrylamide Gel Electrophoresis / Fusion Protein / Full Length Movies / Biochemistry and cell biology / DNA sequence / Cytotoxicity / Biological Sciences / Escherichia coli / Bacteria / Physical sciences / Molecular Biotechnology / Expression / Affinity chromatography / Ion Exchange Chromatography / Enzyme / Protein expression and purification / Enzyme activity / Amino Acid Profile / Molecular Mass / Molecular weight / Protein Kinase / Amino Acid Sequence / Open Reading Frame / Reaction Mechanism / Recombinant Protein / Polyacrylamide Gel Electrophoresis / Fusion Protein / Full Length Movies / Biochemistry and cell biology / DNA sequence

Cloning, Expression, Purification, and Characterization of a Novel Esterase from Lactobacillus plantarum

Technology / High Pressure / Biotechnology / Protein / Glycobiology / Cytotoxicity / Biological Sciences / Escherichia coli / Bacteria / Physical sciences / Molecular Biotechnology / Expression / Affinity chromatography / Ion Exchange Chromatography / Enzyme / Protein expression and purification / Enzyme activity / Amino Acid Profile / Molecular Mass / Molecular weight / Protein Kinase / Amino Acid Sequence / Open Reading Frame / Reaction Mechanism / Recombinant Protein / Polyacrylamide Gel Electrophoresis / Fusion Protein / Full Length Movies / Biochemistry and cell biology / DNA sequence / Cytotoxicity / Biological Sciences / Escherichia coli / Bacteria / Physical sciences / Molecular Biotechnology / Expression / Affinity chromatography / Ion Exchange Chromatography / Enzyme / Protein expression and purification / Enzyme activity / Amino Acid Profile / Molecular Mass / Molecular weight / Protein Kinase / Amino Acid Sequence / Open Reading Frame / Reaction Mechanism / Recombinant Protein / Polyacrylamide Gel Electrophoresis / Fusion Protein / Full Length Movies / Biochemistry and cell biology / DNA sequence

Dengue-1 envelope protein domain III produced in Pichia pastoris: Potential use for serological diagnosis

Dengue Virus / Humans / Dengue / Expression / Molecular cloning / Protein expression and purification / Amino Acid Sequence / Base Sequence / Recombinant Proteins / Enzyme Linked Immunosorbent Assay / Biochemistry and cell biology / immunoglobulin G / Protein expression and purification / Amino Acid Sequence / Base Sequence / Recombinant Proteins / Enzyme Linked Immunosorbent Assay / Biochemistry and cell biology / immunoglobulin G

Expression of a synthetic gene encoding a Tribolium castaneum carboxylesterase in Pichia pastoris

Escherichia coli / Protein Secretion / Animals / Pichia pastoris / Molecular cloning / Protein expression and purification / Tribolium / Specific Activity / Base Sequence / Heterologous Expression / Tribolium Castaneum / Biochemistry and cell biology / Protein expression and purification / Tribolium / Specific Activity / Base Sequence / Heterologous Expression / Tribolium Castaneum / Biochemistry and cell biology

Purification of Recombinant Lecithin:Cholesterol Acyltransferase

Enzyme / Protein expression and purification / Gel electrophoresis / Low molecular weight / Biochemistry and cell biology

Dengue-1 envelope protein domain III produced in Pichia pastoris: Potential use for serological diagnosis

Dengue Virus / Humans / Dengue / Molecular cloning / Protein expression and purification / Amino Acid Sequence / Base Sequence / Recombinant Proteins / Enzyme Linked Immunosorbent Assay / Biochemistry and cell biology / immunoglobulin G / Amino Acid Sequence / Base Sequence / Recombinant Proteins / Enzyme Linked Immunosorbent Assay / Biochemistry and cell biology / immunoglobulin G

Production of soluble, active acetyl serotonin methyl transferase in Leishmania tarentolae

Leishmania / Gene expression / Serotonin / Humans / Escherichia coli / Solubility / Enzyme / Molecular cloning / Protein expression and purification / Protein Expression / Recombinant Proteins / Biochemistry and cell biology / Solubility / Enzyme / Molecular cloning / Protein expression and purification / Protein Expression / Recombinant Proteins / Biochemistry and cell biology

High-Level Bacterial Expression of Human Glutathione Transferase P1-1 Encoded by Semisynthetic DNA

Recombinant DNA Technology / Cell line / Pregnancy / Humans / Placenta / Escherichia coli / Female / Glutathione / Codon Usage / Enzyme / ribosomal RNA / Protein expression and purification / Secondary Structure / Isoenzymes / Protein Expression / Amino Acid Profile / Glutathione Transferase / Base Sequence / Recombinant Protein / Heterologous Expression / Recombinant Proteins / cDNA library / Biochemistry and cell biology / DNA sequence / Escherichia coli / Female / Glutathione / Codon Usage / Enzyme / ribosomal RNA / Protein expression and purification / Secondary Structure / Isoenzymes / Protein Expression / Amino Acid Profile / Glutathione Transferase / Base Sequence / Recombinant Protein / Heterologous Expression / Recombinant Proteins / cDNA library / Biochemistry and cell biology / DNA sequence

Dengue-1 envelope protein domain III produced in Pichia pastoris: Potential use for serological diagnosis

Dengue Virus / Humans / Dengue / Expression / Molecular cloning / Protein expression and purification / Amino Acid Sequence / Base Sequence / Recombinant Proteins / Enzyme Linked Immunosorbent Assay / Biochemistry and cell biology / immunoglobulin G / Molecular Sequence Data / Protein expression and purification / Amino Acid Sequence / Base Sequence / Recombinant Proteins / Enzyme Linked Immunosorbent Assay / Biochemistry and cell biology / immunoglobulin G / Molecular Sequence Data

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